By revealing more of the complex marker distribution on individual cells, we gain a clearer picture of the heterogeneity of this facet of the immune cell compartment as a whole. With this increase in parameter detection per sample, the subsetting of T cells in the literature has exploded.įor example, when considering one aspect of T cell biology, the naïve to memory differentiation post-antigen exposure, the field has transcended from an era of two subsets (naïve and memory) into the era of Central Memory, Transitional Memory, Effector Memory, Terminally Differentiated Effector Memory, etc.Īt first glance, these larger panels bring clarity to the T cell landscape. An antibody panel with more than 10-colors is no longer uncommon, and with the adaptation of the more recent Brilliant dyes, 14+ colors are now very feasible on many instruments. The Benefits And Caveats Of Advanced T Cell Antibody Panelsįlow panel sizes have expanded dramatically in the last 15+ years and continue to do so. Such gating strategies, when paired with CD3 inclusion, doublet exclusion, and appropriate live/dead gating, allow clear, accurate visualization of your T cell population of interest and enumeration of frequency in your sample. Single cell visualization of T cells in a heterogeneous sample is clearest when the defined T cell populations are determined with ‘rock-solid’ gating and data analysis strategies.įor example, detection of the total CD4 and CD8 T cell compartments (via CD3+ CD4+ and CD3+ CD8+ cells, respectively) is straightforward also, T cell populations that are clearly defined by surface antigen expression include antigen-specific (tetramer-binding) memory T cell clones and invariant Natural Killer T (iNKT) cells, a unique T cell subset discerned via binding to a CD1d-glycolipid loaded tetramer. With a large sensitivity range for fluorescent probes, >95% sampling efficiency, and the ability to sort populations of interest for further study, fluorescent-based cytometry remains a tool of choice for T cell analysis. It has been successfully used for many decades to accurately visualize and enumerate a variety of T cell subsets. Flow Cytometry is a remarkably powerful tool for the study of T cells.
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